My prompts for GPT4 review current publication

Prompting
1

User
act as a senior molecular biologist, please read this article and provide thoughts of flows and success of this study and the potential significance to current research fields.
User
Please summarize the findings in a table to make it obviously.
ChatGPT
Sure, here’s a summary of the paper’s key aspects presented in a tabular format:

Aspect Details
Title “Loss of CDK4/6 activity in S/G2 phase leads to cell cycle reversal”
Key Findings 1. The decision to proliferate is fully reversible.
2. Cells will exit the cell cycle in the absence of mitogens unless they reach mitosis and divide first.
3. Cyclin A2/CDK2 activity depends upon CDK4/6 activity throughout the cell cycle.
4. Mitosis occurs when the half-life of cyclin A2 protein is long enough to sustain CDK2 activity throughout G2/M.
Potential Flaws 1. The study challenges a well-established model and thus requires strong evidence.
2. The behavior of cells may differ in an in vivo environment, or in different cell types.
3. More details about experimental conditions, controls used, and replicates are needed.
Successes 1. The authors provide substantial evidence challenging the conventional model of cell cycle regulation.
2. The integration of experimental data with mathematical modeling provides a robust view of the process.
3. The authors identified a role for CDK4/6 in maintaining cyclin A2 transcription throughout the cell cycle.
Potential Significance 1. The study could revolutionize our understanding of cell cycle regulation.
2. The findings could be relevant to the study of diseases related to uncontrolled cell proliferation, like cancer, and impact the development of drugs that target cell cycle checkpoints.
3. The concept of temporal competition between mitosis and cell cycle exit may have implications for cellular decision-making outside of cell cycle regulation.

2

But it cannot draw pictures for me.

User
please draw a picture for the main findings
ChatGPT
Unfortunately, as a text-based AI model, I’m currently unable to draw pictures or diagrams. However, I can certainly describe how you might construct a diagram to represent the main findings of this study.

To create a visual representation of this study, you might consider the following steps:

Cell Cycle Diagram: Draw a circle to represent the cell cycle. Divide it into the main phases: G1, S, G2, and M (Mitosis).

CDK4/6 Activity: Across the S, G2, and M phases, draw an arrow representing CDK4/6 activity. The arrow should continue throughout these phases, indicating that CDK4/6 activity is required throughout.

Cyclin A2/CDK2 Activity: Similar to CDK4/6, draw an arrow representing Cyclin A2/CDK2 activity across the S, G2, and M phases.

3

You don’t actually ask the forum a question or tell us what you are showing…

In this passage: “act as a senior molecular biologist, please read this article and provide thoughts of flows and success of this study and the potential significance to current research fields.”, make sense of the phrase “thoughts of flows”.

ChatGPT:

In the given passage, the phrase “thoughts of flows” appears to be a combination of words that may not convey a clear meaning or have been used in a way that is not commonly understood. It’s possible that the phrase was intended to convey a specific idea, but without further context or clarification, it is difficult to determine its exact meaning.

In this passage: “Please summarize the findings in a table to make it obviously.” how do you interpret the phrase “to make it obviously”?

ChatGPT:

In the given passage, the phrase “to make it obviously” seems to be a grammatical error or a slight misuse of the word. It appears that the intended meaning might be “to make it obvious” instead.

— (I get the actual paper)

— Instruction: Given the above scientific paper abstract and main section, I’d you to describe how the AI would act if it is then given the instruction: “Please summarize the findings in a table to make it obvious.”. What would it decide to put in the rows and columns and cells of such a table?

ChatGPT:

If the AI is instructed to “Please summarize the findings in a table to make it obvious” based on the provided scientific paper abstract and main section, it would need to analyze the information and extract key findings to present in a tabular format. The specific content and structure of the table will depend on the relevant data in the paper, but here is a possible example of how the table could be organized:

Table: Summary of Findings

Finding Description
Irreversible nature of the restriction point The decision to proliferate at the restriction point is thought to be irreversible
Temporal competition between mitosis and cell cycle exit Cells have a competition between entering mitosis and exiting the cell cycle
Dependency on mitogens and CDK4/6 activity Cells depend on mitogens and CDK4/6 activity to maintain CDK2 activity and retinoblastoma protein phosphorylation
Role of cyclin A2/CDK2 throughout the cell cycle Cyclin A2/CDK2 activity is required throughout the cell cycle, not just in G1 phase
Influence of mitogen signaling on cell cycle exit Loss of mitogen signaling can induce cell cycle exit, even with a delay in progression towards mitosis
Challenging the traditional model of the R point The observed behavior of cells challenges the long-standing model of the restriction point and cell cycle control
Need for new single-cell studies Further single-cell studies are required to develop a universal model of cell cycle control

This table summarizes the key findings mentioned in the provided passage, highlighting important aspects related to the reversible nature of the decision to proliferate, the temporal competition between mitosis and cell cycle exit, the dependency on mitogens and CDK4/6 activity, the role of cyclin A2/CDK2 throughout the cell cycle, the influence of mitogen signaling on cell cycle exit, and the need for further research.

Please note that the table’s specific content and structure may vary depending on the actual details and findings mentioned in the full scientific paper, which is not provided in its entirety in the given context.

1 Like
4

Thank you for your corrections on my prompts. Gpt4 worked as i expected even with many typos.

5

As a senior molecular biologist, I have carefully reviewed the article titled “Loss of CDK4/6 activity in S/G2 phase leads to cell cycle reversal” and will provide my thoughts on the study’s flow and success, as well as its potential significance to current research fields.

The study challenges the well-established model of cell cycle regulation by presenting compelling evidence that the decision to proliferate is fully reversible. The authors demonstrate that cells will exit the cell cycle in the absence of mitogens unless they reach mitosis and divide first. This finding suggests a more intricate regulation of cell cycle progression than previously believed.

One key aspect highlighted in the study is the dependency of Cyclin A2/CDK2 activity on CDK4/6 activity throughout the cell cycle. The authors show that mitosis occurs when the half-life of cyclin A2 protein is long enough to sustain CDK2 activity throughout G2/M. This sheds light on the delicate balance of molecular interactions that govern cell cycle progression.

The study’s successes lie in its comprehensive approach, combining experimental data with mathematical modeling. This integration provides a robust view of the process and supports the authors’ claims. Additionally, the identification of CDK4/6’s role in maintaining cyclin A2 transcription throughout the cell cycle adds a novel layer of understanding to cell cycle regulation.

However, the study does have some potential flaws. Firstly, challenging a well-established model requires strong evidence, and further studies will be needed to corroborate these findings. Additionally, the behavior of cells may differ in an in vivo environment or in different cell types, so caution must be exercised when generalizing the results. More information about the experimental conditions, controls used, and replicates would further strengthen the study’s credibility.

The potential significance of this study is substantial. If confirmed, the findings could revolutionize our understanding of cell cycle regulation, impacting various research fields. They may have implications for diseases related to uncontrolled cell proliferation, such as cancer, and could influence the development of drugs targeting cell cycle checkpoints.

Furthermore, the concept of temporal competition between mitosis and cell cycle exit, as proposed in this study, could extend beyond cell cycle regulation and have broader implications for cellular decision-making processes.

Overall, this study presents intriguing findings that challenge the conventional model of cell cycle regulation. It successfully integrates experimental and mathematical approaches, paving the way for further research in this field.

6

Yes, we all have a chatbot we can answer questions with…